课题组结合肿瘤分子生物学、RNA生物学和生物信息学，致力于肿瘤诊疗新靶点的发现和作用研究，建立了相关的RNA测序、生物信息方法和数据库（exLR-seq, ASJA, EV-origin, exoRBase, RJunBase），提出并发现癌症中存在丰富的肿瘤特异转录本TSTs (Nat Commun, 2016; Cell Rep, 2018; Hepatology, 2019; Cancer Res, 2020; Nucleic Acids Res, 2021)；解析了外泌体/细胞外囊泡RNA组分及其在癌症中的应用(Cell Res, 2015; Nucleic Acids Res, 2018&2022; Clin Chem, 2019; Gut, 2020)。课题组目前重点开展肿瘤标志物和核酸药物（小核酸和mRNA）研究。迄今发表SCI论文60余篇，H-index 45，被引超过1万次，申请发明专利三项，获得国家重点研发课题、国家自然科学基金、上海市科技创新项目，以及国家“万人计划”青年拔尖人才、上海市浦江和启明星计划资助。

1.Hongyan Lai, Yuchen Li, Hena Zhang, Jia Hu, Jiatao Liao, Ying Su, Qin Li, Bing Chen, Caiping Li, Zhen Wang, Yan Li, Jialei Wang, Zhiqiang Meng^#, Zhaohui Huang^#, Shenglin Huang^#. exoRBase 2.0: an atlas of mRNA, lncRNA and circRNA in extracellular vesicles from human biofluids. Nucleic Acids Research. 2022;50(D1):D118-D128.

2.Li Y, Chen B, Zhao J, Li Q, Chen S, Guo T, Li Y, Lai H, Chen Z, Meng Z, Guo W^#, He X^#, Huang S^#, HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein. Advanced Science. 2021, 2001701.

3.Qin Li, Hongyan Lai, Yuchen Li, Bing Chen, Siyuan Chen, Yan Li, Zhaohui Huang, Zhiqiang Meng, Peng Wang, Zhixiang Hu^#, Shenglin Huang^#. RJunBase: a database of RNA splice junctions in human normal and cancerous tissues. Nucleic Acids Research. 2021;49(D1):D201-D211.

4.Qiupeng Zheng, Jingjing Zhao, Hui Yu, Huajie Zong, Xigan He, Yiming Zhao, Yan Li, Yu Wang, Yichao Bao, Yuchen Li, Bing Chen, Weijie Guo, Yilin Wang, Zhiao Chen, Yingjun Zhao, Lu Wang^#, Xianghuo He^#, Shenglin Huang^#. Tumor-specific Transcripts are Frequently Expressed in Hepatocellular Carcinoma with Clinical Implication and Potential Function. Hepatology. 2020;71(1):259-274.

5.Yu S, Li Y, Liao Z, Wang Z, Wang Z, Li Y, Qian L, Zhao J, Zong H, Kang B, Zou WB, Chen K, He X, Meng Z, Chen Z^#, Huang S^#, Wang P^#. Plasma extracellular vesicle long RNA profiling identifies a diagnostic signature for the detection of pancreatic ductal adenocarcinoma. Gut. 2020;69(3):540-550.

6.Yuchen Li, Jingjing Zhao, Shulin Yu, Zhen Wang, Xigan He, Yonghui Su, Tianan Guo, Haoyue Sheng, Jie Chen, Qiupeng Zheng, Yan Li, Weijie Guo, Xiaohong Cai, Guohai Shi, Jiong Wu, Lu Wang, Peng Wang^#, Xianghuo He^#, Shenglin Huang^#. Extracellular vesicles long RNA sequencing reveals abundant mRNA, circRNA and lncRNA in human blood as biomarkers for cancer diagnosis. Clinical Chemistry. 2019;65(6):798-808.

7.Xiu B, Chi Y, Liu L, Chi W, Zhang Q, Chen J, Guo R, Si J, Li L, Xue J, Shao ZM, Wu ZH^#, Huang S^#, Wu J^#. LINC02273 drives breast cancer metastasis by epigenetically increasing AGR2 transcription. Mol Cancer. 2019;18(1):187.

8.Jingjing Zhao, Qin Li, Yuchen Li, Xianghuo He, Qiupeng Zheng^#, Shenglin Huang^#. ASJA: Assembling Splice Junctions Analysis. Computational and Structural Biotechnology Journal. 2019;17:1143-1150.

9.Guo W, Hu Z, Bao Y, Li Y, Li S, Zheng Q, Lyu D, Chen D, Yu T, Li Y, Zhu X, Ding J, Zhao Y, He X^#, Huang S^#. A LIN28B Tumor-Specific Transcript in Cancer. Cell Reports. 2018; 22(8):2016-2025.

10.Shengli Li, Yuchen Li, Bing Chen, Jingjing Zhao, Shulin Yu, Yan Tang, Qiupeng Zheng, Yan Li, Peng Wang, Xianghuo He, Shenglin Huang^#. exoRBase: a database of circRNA, lncRNA and mRNA in human blood exosomes. Nucleic Acids Research. 2018;46:D106–D112.

11.Qiupeng Zheng, Chunyang Bao, Weijie Guo, Shuyi Li, Jie Chen, Bin Chen, Yanting Luo, Dongbin Lyu, Yan Li, Guohai Shi, Linhui Liang, Jianren Gu, Xianghuo He^#, Shenglin Huang^#. Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAs. Nature Communications. 2016; 7:11215.

12.Yan Li, Qiupeng Zheng, Chunyang Bao, Shuyi Li, Weijie Guo, Jiang Zhao, Di Chen, Jianren Gu, Xianghuo He^#, Shenglin Huang^#. Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis. Cell Research. 2015;25(8):981-4.