Haojie Lu, Ph.D.

Education

• B.S. 1992.9-1996.7, Xiamen University, China

• Ph.D. 1996.9-2001.7, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences

Work Experience

• Postdoc training, 2001.9-2003.7, Shanghai Insititute of Organic Chemistry, Chinese Academy of Sciences

• Assistant Professor, 2003.9-2003.12, Department of Chemistry, Fudan University

• Associate Professor, 2004.1-2008.11, Department of Chemistry, Fudan University

• Professor, 2008.12-, Institutes of Biomedical Sciences, Department of Chemistry, Fudan University

• Visiting Scholar, 2006.9-2007.9, School of Medicine, University of California, Los Angeles (UCLA)

Contact

Institutes of Biomedical Sciences, Fudan University, Shanghai 200032 Tel: +86 021-54237618

Email: luhaojie@fudan.edu.cn

Research Areas

• Proteomics

• Glycomics

• Mass spectrometry-based analytical methods

• Disease biomarker

Research Interest

Our lab focuses on the development of new analytical methods and technologies for proteome and glycome research based on mass spectrometry with the interdisciplinary integration of analytical chemistry, materials science and systems biology, which including 1)Development of novel MS-based approaches for PTM-proteome research including protein glycosylation, protein termini, palmitoylation and other types of PTM. 2) Application of MS-based proteomics and glycomics in research of human diseases.

Awards and Honors

2005 The First Prize in Natural Science Award of the Ministry of Education（Rank Fourth）

2006 Program for New Century Excellent Talents in University of Ministry of

Education of China

2006 Shanghai Science and Technology Committee Rising-Star Program;

2008 Meiji Life Sciences Awards (Outstanding)

2009 Young Chemistry Award of Chinese Chemical Society

2010 National Science Fund for Distinguished Young Scholars

2014 Ministry of Science and Technology Innovation Talent Promotion Plan

2014 The Second Prize of Outstanding Achievements in Scientific Research of Higher Education Institutions of the Ministry of Education (Rank Second)

2016 The “10,000 plan” Leading Talent Program

Selected Publications

1. Sun Zhenyu, Fu Bin, Wang Guoli, Zhang Lei, Xu Ruofan, Zhang Ying*, Lu Haojie* High-throughput site-specific N-glycoproteomics reveals glyco-signatures for liver disease diagnosis. Nat. Sci. Rev., 2022, DOI: 10.1093/nsr/nwac059

2. Linlin Wu, Fei Weiwei, Liu Zhiyong, Zhang Lei, Fang Caiyun*, and Lu Haojie* Specific and reversible enrichment of early-stage glycated proteome based on thiazolidine chemistry and palladium-mediated cleavage. Anal Chem, 2022, 94, 13, 5213-5220.

3. Cheng Mengxia, Shu Hong, Yang Maohua, Yan Guoquan, Zhang Lei, Wang Liang, Wang Wenning, and Lu Haojie* Fast discrimination of sialylated N-Glycan linkage isomers with one-step derivatization by microfluidic capillary electrophoresis-mass spectrometry. Anal Chem, 2022, 94, 11, 4666–4676.

4. Wang Zhongjie, Yuan Wenjuan, Zhang Lei, Zhang Ying, Lu Haojie* NAPT, an unbiased approach for sequential analysis of protein N- and C- terminome. Chem Commun, 2022, 58, 9397-9400

5. Wu Linlin, Fang Caiyun, Zhang Lei, Yuan Wenjuan, Yu Xiaofang*, Lu Haojie* Integrated strategy for discovery and validation of glycated candidate biomarkers for hemodialysis patients with cardiovascular complications. Anal Chem, 2021, 93,4398−4407

6. Cheng Mengxia, Shu Hong, Peng Ye, Feng Xiaoxiao, Yan Guoquan, Zhang Lei, Yao Jun, Bao Huimin, Lu Haojie* Specific analysis of α-2,3-sialylated N-Glycan linkage isomers by microchip capillary electrophoresis-mass Spectrometry. Anal Chem, 2021, 93, 5537–5546

7. Shao Yuyin, Bao Huimin*, Ma Lixiang, Yuan Wenjuan, Zhang Lei, Yao Jun, Meng Peiyi, Peng Ye, Zhang Siwen, Cao Ting, Lu Haojie* Enhancing comprehensive analysis of newly synthesized proteins based on cleavable bioorthogonal tagging. Anal. Chem., 2021, 93, 27, 9408-9417

8. Wang Zhongjie, Zhang Lei, Yuan Wenjuan, Zhang Ying *, Lu Haojie* SAPT, a fast and efficient approach for simultaneous profiling of protein N- and C-terminome. Anal Chem, 2021, 93, 30, 10553–10560.

9. Feng Xiaoxiao, Shu Hong, Zhang Shu, Peng Ye, Zhang Lei, Cao Xinyi, Wei Liming* and Lu Haojie* Relative Quantification of N-Glycopeptide Sialic Acid Linkage Isomers by Ion Mobility Mass Spectrometry. Anal Chem, 2021,93, 47, 15617-15625

10. Du Xiaoxian, Lu Jingtian, Yang Lijun, Bao Huimin, Zhang Lei, Yan Guoquan, Fang Caiyun*, Lu Haojie* Rapid and Easy Enrichment Strategy for Naturally Acetylated N-Termini Based on LysN Digestion and Amine-reactive Resin Capture. Analytical Chemistry, 2020, 92, 12, 8315–8322

11. Di Yi, Zhang Lei, Zhang Ying, Zhao Huanhuan, Yan Guoquan, Yao Jun, Zhang Shu and Lu Haojie*, MdCDPM: A Mass Defect-Based Chemical-Directed Proteomics Method for Targeted Analysis of Intact Sialylglycopeptides. Analytical Chemistry, 2019, 91, 9986-9992.

12. Du Xiaoxian, Fang Caiyun, Yang Lijun, Bao Huimin, Zhang Lei, Yan Guoquan and Lu Haojie*, In-Depth Analysis of C Terminomes Based on LysC Digestion and Site-Selective Dimethylation. Analytical Chemistry, 2019, 91, 6498-6506.

13. Yang Lijun, Du Xiaoxian, Peng Ye, Cai Yan, Wei Lei, Zhang Ying* and Lu Haojie*, Integrated Pipeline of Isotopic Labeling and Selective Enriching for Quantitative Analysis of N-Glycome by Mass Spectrometry. Analytical Chemistry, 2019, 91, 1486-1493.

14. Zhang Siwen, Fang Caiyun, Yuan Wenjuan, Zhang Ying, Yan Guoquan, Zhang Lei, Di Yi, Cai Yan and Lu Haojie*, Selective Identification and Site-Specific Quantification of 4-Hydroxy-2-nonenal-Modified Proteins. Analytical Chemistry, 2019, 91, 5235-5243.

15. Yang Lijun, Sun Zhenyu, Lei Zhang, Yan Cai, Ye Peng, Ting Cao, Ying Zhang* and Lu Haojie*. Chemical labeling for fine mapping of IgG Nglycosylation by ETD-MS. Chem. Sci., 2019, 10, 9302-9307

16. Yang Lijun, Peng Ye, Jiao Jing, Tao Tao, Yao Jun, Zhang Ying*, LuHaojie*.Metallic element chelated tag labeling (MeCTL) for quantitation of N-Glycans in MALDI-MS. Analytical Chemistry. 2017, 89, 7470–7476.

17. Tong Qinghe, Yan Tianyang, Tao Tao, Zhang Lei, Xie Liqi* and LuHaojie*. Reductive amination combining dimethylation for quantitative analysis of early-stage glycated proteins. Analytical Chemistry, 2018, 90, 3752−3758.

18. Wei Lei, Cai Yan, Yang Lijun, Zhang Ying * andLu Haojie*. Duplex stable isotope labeling (DuSIL) for simultaneous quantitation and distinction of sialylated and neutral NGlycans by MALDI-MS. Analytical Chemistry, 2018, 90,10442−10449.

19. Zhang Ying, Peng Ye, Yang Lijun, LuHaojie*, Advances in sample preparation strategies for MS-based qualitative and quantitative N-glycomics. Trends in Analytical Chemistry, 2018, 99:34-46.

20. Zhang Xiaoqin, Zhang Yuting, Fang Caiyun*, Zhang Lei, Yang Pengyuan, Wang Changchun and Lu Haojie*. Ultradeep palmitoylomics enabled by dithiodipyridine-functionalized magnetic nanoparticles. Analytical Chemistry,2018, 90, 6161−6168.

21. Zhang Xiaoqin, Zhang Lei, Ji Guanghui, Lei Qunying, Fang Caiyun * and LuHaojie*. Site-specific quantification of protein palmitoylation by cysteine-stable isotope metabolic labeling. Analytical Chemistry, 2018, 90,10543−10550.

22. Di Yi, Zhang Ying, Zhang Lei, Tao Tao, Lu Haojie* MdFDIA: A mass defect based four-plex data-independent acquisition strategy for proteome quantification. Analytical Chemistry. 2017, 89, 10248–10255.

23. Cao Ting, Zhang Lei, Zhang Ying, Yan Guoquan, Fang Caiyun, Bao Huimin, Lu Haojie*. Site-specific quantification of protein ubiquitination on MS2 fragment ion level via isobaric peptide labeling. Analytical Chemistry. 2017, 89, 11468-11475.

24. Yuan Wenjuan, Zhang Ying, Xiong Yun, Tao Tao, Wang Yi, Yao Jun, Zhang Lei, Yan Guoquan, Bao Huimin, Lu Haojie*. Highly selective and large scale mass spectrometric analysis of 4-Hydroxynonenal modification via fluorous derivatization and fluorous solid-phase extraction. Analytical Chemistry. 2017, 89, 3093-3100.

25. Lu Haojie, Zhang Ying, Yang Pengyuan* Advancements in mass-spectrometry based glycoproteomics and glycomics. National Science Reviews, 2016, 3, 345-364.

26. Tong Qinghe, Tao Tao, Xie Liqi*, Lu Haojie*.  ELISA-PLA: A novel hybrid platform for the rapid, highly sensitive and specific quantification of proteins and post-translational modifications. Biosensors & Bioelectronics, 2016, 80, 385-391.

27. Li Lulu, Jiao Jing, Cai Yan, Zhang Ying*, Lu Haojie*. Fluorinated Carbon Tag Derivatization Combined with Fluorous Solid-Phase Extraction: A New Method for the Highly Sensitive and Selective Mass Spectrometric Analysis of Glycans. Analytical Chemistry. 2015, 87, 5125-5131.

28. Zhang Ying, Zhang Cheng, Jiang Hucong, Yang Pengyuan, Lu Haojie*. Fishing the PTM proteome with chemical approaches using functional solid phases. Chemical Society Reviews. 2015, 44, 8260-8287.

29. Li Wei, Liu Minbo, Feng Shanshan, Li Xiaomin, Wang Jinxiu, Shen Dengke, Li Yuhui, Sun Zhenkun, Elzatahry Ahmed A., Lu Haojie*, Zhao Dongyuan* Template-free synthesis of uniform magnetic mesoporous TiO₂ nanospindles for highly selective enrichment of phosphopeptides. Materials Horizons. 2014, 1, 439-445.

30. Liu Liting, Yu Meng, Zhang Ying, Wang Changchun*, Lu Haojie* Hydrazide Functionalized Core-Shell Magnetic Nanocomposites for Highly Specific Enrichment of N-Glycopeptides.Acs Applied Materials & Interfaces. 2014, 6, 7823-7832.

31. Wang Yali, Liu Minbo, Xie Liqi, Fang Caiyun, Xiong Huanming*, Lu Haojie*. Highly Efficient Enrichment Method for Glycopeptide Analyses: Using Specific and Nonspecific Nanoparticles Synergistically. Analytical Chemistry. 2014, 86, 2057-2064.

32. Zhang Ying, Ma Wanfu, Zhang Cheng, Wang Changchun*,Lu Haojie*.Titania Composite Microspheres Endowed with a Size-Exclusive Effect toward the Highly Specific Revelation of Phosphopeptidome. Acs Applied Materials & Interfaces. 2014, 6, 6290-6299.

33. Zhang Ying, Yu Meng, Zhang Cheng, Ma Wanfu, Zhang Yuting, Wang Changchun*, Lu Haojie*. Highly Selective and Ultra Fast Solid-Phase Extraction of N-Glycoproteome by Oxime Click Chemistry Using Aminooxy-Functionalized Magnetic Nanoparticles. Analytical Chemistry. 2014, 86, 7920-7924.

34. Zhang Ying, Kuang Min, Zhang Lijuan, Yang Pengyuan, Lu Haojie*. An Accessible Protocol for Solid-Phase Extraction of N-Linked Glycopeptides through Reductive Amination by Amine-Functionalized Magnetic Nanoparticles. Analytical Chemistry. 2013, 85, 5535-5541.

35. Liu Minbo, Zhang Lijuan, Zhang Lei, Yao Jun, Yang Pengyuan,Lu Haojie*. Approach for Identification and Quantification of C-Terminal Peptides: Incorporation of Isotopic Arginine Labeling Based on Oxazolone Chemistry. Analytical Chemistry. 2013, 85 (22), 10745-10753.

36. Ma Wanfu, Zhang Ying, Li Lulu, You Lijun, Zhang Peng, Zhang Yuting, Li Jumei, Yu Meng, Guo Jia, Lu Haojie*, Wang Changchun*. Tailor-Made Magnetic Fe₃O₄@mTiO(₂) Microspheres with a Tunable Mesoporous Anatase Shell for Highly Selective and Effective Enrichment of Phosphopeptides. ACS Nano. 2012, 6, 3179-3188.

37. Nie Aiying, Zhang Lei, Yan Guoquan, Yao Jun, Zhang Yang, Lu Haojie*, Yang Pengyuan, He Fuchu. In Vivo Termini Amino Acid Labeling for Quantitative Proteomics. Analytical Chemistry. 2011, 83, 6026-6033.

38. Xu Yawei, Wu Zhangxiong, Zhang Lijuan,Lu Haojie*, Yang Pengyuan. Webley Paul A., Zhao Dongyuan*, Highly Specific Enrichment of Glycopeptides Using Boronic Acid-Functionalized Mesoporous Silica. Analytical Chemistry. 2009, 81, 503-508.

39. Xiong Huanming, Guan Xiaoyan, Jin Linghua, Shen Wenwen, Lu Haojie*, Xia Yongyao*. Surfactant-free synthesis of SnO₂@PMMA and TiO₂@PMMA core-shell nanobeads designed for peptide/protein enrichment and MALDI-TOF MS analysis. Angewandte Chemie-International Edition. 2008, 47, 4204-4207.

40. CaCO₃-poly (methyl methacrylate) nanoparticles for fast enrichment of low-abundance peptides followed by CaCO₃-core removal for MALDI-TOF MS analysis. Jia Weitao, Chen Xuehua, Lu Haojie*, Yang Pengyuan*. Angewandte Chemie-International Edition. 2006, 45, 3345-3349.