Education & Training Work

Nov. 2020-Present Professor, Principal Investigator

Institutes of Biochemical Sciences, Shanghai Cancer Center, Shanghai Medical College, Fudan University

Feb. 2015-Oct. 2020 Postdoc fellow (Mentor: Prof. Chuan He)

Department of Chemistry, University of Chicago, Chicago, USA

Sep. 2009-Jul. 2014 Ph.D. (Mentor: Prof. Yanhui Xu)

Biochemistry and molecular biology, Fudan University

Sep. 2005- Jul. 2009 B.S.

Biological Science, Soochow University

Contact

Institutes of Biomedical Sciences, Shanghai Cancer Center,

Shanghai Medical College Fudan University,

Tel: +86 18001742360

E-mail: luluhu@fudan.edu.cn

Research Areas：

We aim at developing epigenomic and epitranscriptomic profiling tools at single cell and spatial scale.

Selected Publications

1: Hu L*^#, Liu S*, Peng Y*, Ge R*, Su R*, Senevirathne C, Harada BT, Dai Q, Wei J, Zhang L, Hao Z, Luo L, Wang H, Wang Y, Luo M, Chen M^#, Chen J^#, He C^#.(*represents equal contribution, ^#represents corresponding author.)

m⁶A RNA modifications are measured at single-base resolution across the mammalian transcriptome. Nat Biotechnol. 2022 Aug;40(8):1210-1219. doi: 10.1038/s41587-022-01243-z. Epub 2022 Mar 14.

2: Hu L*, Liu Y*, Han S*, Yang L*, Cui X, Gao Y, Dai Q, Lu X, Kou X, Zhao Y, Sheng W, Gao S, He X, He C. Jump-seq: Genome-Wide Capture and Amplification of 5-Hydroxymethylcytosine Sites. J Am Chem Soc. 2019 Jun 5;141(22):8694-8697. doi: 10.1021/jacs.9b02512. Epub 2019 May 23.

3: Zhang LS, Liu C, Ma H, Dai Q, Sun HL, Luo G, Zhang Z, Zhang L, Hu L, Dong X, He C. Transcriptome-wide Mapping of Internal N7-Methylguanosine Methylome in Mammalian mRNA. Mol Cell. 2019 Jun 20;74(6):1304-1316.e8. doi: 10.1016/j.molcel.2019.03.036. Epub 2019 Apr 25.

4: Ji L, Jordan WT, Shi X, Hu L, He C, Schmitz RJ. TET-mediated epimutagenesis of the Arabidopsis thaliana methylome. Nat Commun. 2018 Mar 1;9(1):895. doi: 10.1038/s41467-018-03289-7.

5: Wang W, Xu L, Hu L, Chong J, He C, Wang D. Epigenetic DNA Modification N6-Methyladenine Causes Site-Specific RNA Polymerase II Transcriptional Pausing. J Am Chem Soc. 2017 Oct 18;139(41):14436-14442. doi: 10.1021/jacs.7b06381. Epub 2017 Oct 3.

6: Lu J, Hu L*, Cheng J, Fang D, Wang C, Yu K, Jiang H, Cui Q, Xu Y, Luo C. A computational investigation on the substrate preference of ten-eleven-translocation 2 (TET2). Phys Chem Chem Phys. 2016 Feb 14;18(6):4728-38. doi: 10.1039/c5cp07266b. (*represents equal contribution).

7: Hu L, He C. Detecting hepatocellular carcinoma in blood. Cell Res. 2015 Dec;25(12):1279-80. doi: 10.1038/cr.2015.136. Epub 2015 Nov 24.

8: Hu L*, Lu J*, Cheng J*, Rao Q, Li Z, Hou H, Lou Z, Zhang L, Li W, Gong W, Liu M, Sun C, Yin X, Li J, Tan X, Wang P, Wang Y, Fang D, Cui Q, Yang P, He C, Jiang H, Luo C, Xu Y. Structural insight into substrate preference for TET-mediated oxidation. Nature 2015 Nov 5;527(7576):118-22. (*represents equal contribution).

9: Hu L*, Li Z*, Cheng J*, Rao Q, Gong W, Liu M, Shi YG, Zhu J, Wang P, Xu Y. Crystal structure of TET2-DNA complex: insight into TET-mediated 5mC oxidation. Cell 2013 Dec 19;155(7):1545-55 (*represents equal contribution).

10: Hu L*, Li Z*, Wang P, Lin Y, Xu Y. Crystal structure of PHD domain of UHRF1 and insights into recognition of unmodified histone H3 arginine residue 2. Cell Res. 2011 Sep;21(9):1374-8 (*represents equal contribution).

11: Rajakumara E*, Wang Z*, Ma H, Hu L, Chen H, Lin Y, Guo R, Wu F, Li H, Lan F, Shi YG, Xu Y, Patel DJ, Shi Y. PHD finger recognition of unmodified histone H3R2 links UHRF1 to regulation of euchromatic gene expression. Mol. Cell 2011 Jul 22;43(2):275-84 (*represents equal contribution).

12: Yang Y*, Hu L*, Wang P*, Hou H, Lin Y, Liu Y, Li Z, Gong R, Feng X, Zhou L, Zhang W, Dong Y, Yang H, Lin H, Wang Y, Chen CD, Xu Y.Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans. Cell Res. 2010 Aug;20(8):886-98 (*represents equal contribution).

Patents:

Methods for detecting cytosine modification.
m⁶A-SAC-seq for quantitative RNA m⁶A sequencing.