课题组长
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  • 王文宁

    博士,教授,博士生导师
    地址:邯郸路 220号,老化学楼 206室,上海 200433
    电话:021-65643985 (办公室),021-65643792 (实验室)
    传真:021-65641740
    邮箱:wnwang@fudan.edu.cn
    实验室主页:http://homepage.fudan.edu.cn/wenningwang/home/

工作经历
教授,复旦大学,化学系,(2005.4-今)
访问学者,香港科技大学,生物化学系,(2002.2-2003.8)
副教授,复旦大学,化学系,(2000.12-2005.4)
讲师,复旦大学,化学系,(1996.4-2000.12)
博士后,日本国立分子科学研究所,(1995.12-1996.3)
教育经历
博士,复旦大学,化学系,(1990-1995)
学士,复旦大学,化学系,(1986-1990)
所获人才项目
曙光学者,上海市教委,2004
香港求槎基金会内地青年学者,香港求槎基金会,2003
研究方向
课题组主要运用结构生物学和计算生物学方法研究蛋白质的结构、动力学性质与生物功能的关系。我们主要关注的问题包括膜蛋白的大范围构像变化动力学,翻译后修饰对蛋白质构像的调节,信号传导通路中的蛋白-蛋白相互作用,以及内在无序蛋白的构象动力学与功能等。研究手段包括液体核磁共振,X-射线晶体衍射,单分子荧光能量共振转移,分子动力学模拟等计算生物学方法,及生物化学方法等。具体的研究方向包括:膜相关蛋白构像变化的动力学和调控;干细胞非对称分裂调控机制的研究;结合多种生物物理手段研究内在无序蛋白的结构、动力学和功能的关系。
课题组长王文宁主持和参与多项国家和省部级项目,包括国家重点研发计划、科技部973,863项目、国家自然科学基金面上项目、教育部博士点基金、上海市科委项目等。迄今为止,已在国内外学术刊物上发表论文近90篇,包括Molecular Cell, Nature Communication, J. Am. Chem. Soc., Angew. Chem. Int. Ed, J. Biol. Chem.等。
代表论文
  1. Zhu, J., Wen, W., Zheng, Z., Shang, Y., Wei, Z., Xiao, Z., Pan, Z., Du Q.*, Wang W.*, Zhang M.* (2011) “LGN/mInsc and LGN/NuMA complexes structures suggest distinct functions in asymmetric cell division for the Par3/mInsc/LGN and Gai/LGN/NuMA pathways”, Mol. Cell, 43, 418-431.

  2. Jia, M., Shan, Z., Yang, Y., Liu, C., Li, J., Luo, Z., Zhang, M., Cai Y.*, Wen W.*, Wang W.* (2015) “The structural basis of Miranda-mediated Staufen localization during Drosophila neuroblast asymmetric division” Nat. Commun. 6:8381.

  3. Wang, W.*, Weng, J., Zhang, X., Liu, M., Zhang, M.* (2009) “Creating conformational entropy by increasing inter-domain mobility in ligand binding regulation: a revisit to N-terminal tandem PDZ domains of PSD-95”, J. Am. Chem. Soc. 131, 787-796.

  4. Fu, H., Liu, Z., Li, Z., Wang, W.*, Fan K.* (2006) “Periodic density functional theory study of propane oxidative dehydrogenation over V2O5 (001) surface”, J. Am. Chem. Soc. 128, 11114-11123.

  5. Feng, Y., Zhang, L., Wu, S., Gao, X., Liu, J.*, Huang, X.*, Wang, W.* (2016) “Conformational Dynamics of apo-GlnBP Revealed by Experimental and Computational Analysis” Angew. Chem. Int. Ed. Engl. 55, 13990-13994.

  6. Yao, C., Wei, C., Huang, Z., Lu, Y., El-Toni, AM, Ju, D., Zhang, X., Wang, W.*, Zhang, F.* (2016) “Phosphorylated Peptides Functionalization of Lanthanide Upconversion Nanoparticles for Tuning the Nanomaterial-Cell Interaction” ACS Appl. Mater. Interfaces, 8, 6935-6943.

  7. Ma, W., Shang, Y., Wei, Z., Wen, W., Wang, W.*, Zhang, M.* (2010) “Phosphorylation of DCC by ERK2 is Facilitated by Direct Docking of the Receptor P1 Domain to the Kinase” Structure, 18, 1502-1511.

  8. Pan, Z., Zhu, J., Shang, Y., Wei, Z., Jia, M., Xia, C., Wen, W., Wang W.*, Zhang M.* (2013) “An auto-inhibited conformation of LGN reveals a distinct interaction mode between GoLoco motifs and TPR motifs” Structure, 21, 1007-1017.

  9. Weng, J., Fan, K. Wang, W.* (2010) “The conformational transition pathway of ATP binding cassette transporter MsbA revealed by atomistic simulations” J. Biol. Chem. 285, 3053-3063.

  10. Jia, M., Li, J., Zhu, J., Wen, W., Zhang, M., Wang, W.* (2012) “Crystal Structures of the scaffolding protein LGN reveal the general mechanism by which GoLoco binding motifs inhibit the release of GDP from Gai subunits in G-coupled heterotrimeric proteins”, J. Biol. Chem., 287, 36766-36776.

  11. Zuo, Z., Wang, B., Weng, J.*, Wang, W.* (2015) “Stepwise substrate translocation mechanism revealed by free energy calculations of doxorubicin in the multidrug transporter AcrB.” Sci. Rep. 5:13905.

  12. Weng, J., Ma, J., Fan, KN, Wang, W.* (2008) “The Conformational Coupling .and Translocation Mechanism of Vitamin B12 ATP-binding Cassette (ABC) Transporter BtuCD”, Biophys. J., 94, 612-62.

  13. Weng, J., Ma, J. Fan, K., and Wang, W.* (2009) “Asymmetric conformational flexibility in the ATP-Binding Cassette transporter HI1470/1” Biophys. J. 96, 1918-1930.

  14. Weng, J., Gu, S., Gao, X., Huang, X.*, Wang, W.* (2017) “Maltose Binding Protein Effectively Stabilizes the Partially Closed Conformation of the ATP-binding Cassette Transporter MalFGK2” Phys. Chem. Chem. Phys. doi: 10.1039/C6CP07943A.

  15. Wang, B., Weng, J.*, Wang, W.* (2015) “Substrate binding accelerates the conformational transitions and substrate dissociation in multidrug efflux transporter AcrB” Front. Microbiol. 6, 302.

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